賴金美老師

賴金美老師

職稱: 
副教授
研究室名稱: 
訊息傳遞研究室
辦公室位置: 
耕莘樓生命科學系館 LS303
連絡電話: 
(02)2905-3595
電子信箱: 
062989@mail.fju.edu.tw
學歷: 
台大醫學院微生物所碩士
台大醫學院生化分生所博士
專長: 
細胞生物學
分子生物學
生物化學
研究方向: 
1)探討 TOPK 基因於化學治療所扮演之角色及其於肺癌治療之應用
2)以具 Tcf/Lef 冷光報告基因之肝癌細胞平台篩選 Wnt/β-catenin 訊息之抑制劑,並探討其作用機制及評估其於癌症幹細胞治療之應用。
3)石蓮花萃取物及生物相仿物之保肝及抗肝癌之研究。

期刊論文

  1. Lai JM、Hsieh CL、Chang ZF,「Caspase activation during phorbol ester-induced apoptosis ROCK-dependent myosin-mediated contraction.」,Journal Cell Science.卷,期(2003-09-01):3491-3501(SCI)
  2. Lai JM、Lu CY、 Yang-Yen HF、Chang ZF,「Lysophosphatidic acid promotes phorbol-ester-induced apoptosis in TF-1 cells by interfering with adhesion.」,Biochemical Journal.卷,期(2001-10-01):227-233(SCI)
  3. Lai JM、Wu S、Huang DY、Chang ZF,「Cytosolic retention of phosphorylated extracellular signal-regulated kinase and a Rho-associated kinase-mediated signal impair expression of p21(Cip1/Waf1) in phorbol 12-myristate-13-acetate-induced apoptotic cells.」,Molecular and Cell Biology卷,期(2002-11-01):7581-7592(SCI)
  4. Lin YS、Su LJ、Yu CTR、Wong FH、Yeh HH、Chen SL、Wu JC、Lin WJ、Lai JM、Huang CYF,「Gene Expression Profiles of the Aurora Family Kinases.」,Gene expression13卷,1期(2006-03-01):15-26(SCI)
  5. Chen KY、Lee YC、Lai JM、Chang YL、Lee YC、 Yu CJ、Huang CYF、Yang PC,「Identification of trophinin as an enhancer for cell invasion and a prognostic factor for early stage lung cancer.」,Eur J Cancer卷,43期(2007-03-01):782-790(SCI)
  6. Hsu CN、Lai JM、Liu CH、Tseng HH、Lin CY、Lin KT、Yeh HH、Sung TY、Hsu WL、Huang CYF,「Detection of the inferred interaction network in hepatocellular carcinoma from EHCO (Encyclopedia of Hepatocellular Carcinoma genes Online」,BMC Bioinformatics.卷,8期(2007-02-01):(SCI)
  7. Yang CY、Chang CH、Yu YL、Lin TSE、Lee SA、Yen CC、Yang JM、Lai JM、Hong YR、Huang CYF,「PhosphoPOINT: a comprehensive human kinase interactome and phospho-protein database」,Bioinformatics24卷,期(2008-09-01):i14-i20(SCI)
  8. Wong FH、Huang CYF、Su LJ、Wu YC、Lin YS、Hsia JY、Tsai HT、Lee SA、Lin CH、Lai JM,「Combination of microarray profiling and protein-protein interaction databases delineates the minimail discriminators as a metastasis network for esophageal squamous cell carcinoma」,INTERNATIONAL JOURNAL OF ONCOLOGY34卷,期(2009-06-01):117-128(SCI)
  9. Lee SA、Chan CH、Tsai CH、Lai JM、Wang FS、Kao CY、Huang CYF,「Ortholog-based protein-protein interaction predication and its application to inter-species interactions」,BMC Bioinformatics9卷,12期(2008-12-01):S11(SCI)
  10. Chen CH、Lai JM、Chou TY、Chen CY、Su LJ、Lee YC、Cheng TS、Hong YR、Chou CK、Huang CY,「VEGFA upregulates FLJ10540 and modulates migration and invasion of lung cancer via PI3K/AKT pathway. 」,PLoS One4卷,4期(2009-04-01):e5052(SCI)
  11. Lee SA、Chan CH、Chen TC、Yang CY、Huang KC、Tsai CH、Lai JM、Wang FS、Kao CY、Huang CY,「POINeT: protein interactome with sub-network analysis and hub prioritization.」,BMC Bioinformatics.10卷,期(2009-04-01):114(SCI)
  12. Chen TC、Lee SA、Chan CH、Juang YL、Hong YR、Huang YH、Lai JM、Kao CY、Huang CY,「Cliques in mitotic spindle network bring kinetochore-associated complexes to form dependence pathway.」,Proteomics.9卷,16期(2009-08-01):4048-4062(SCI)
  13. Lai JM、Chang JT、Wen CL、Hsu SL,「Emodin induces a reactive oxygen species-dependent and ATM-p53-Bax mediated cytotoxicity in lung cancer cells.」,Eur J Pharmacol.623卷,1-3期(2009-11-01):1-9(SCI)
  14. Chen TC、Lee SA、Hong TM、Shih JY、Lai JM、Chiou HY、Yang SC、Chan CH、Kao CY、Huang CY,「From midbody protein-protein interaction network construction to novel regulators in cytokinesis.」,J. Proteome Res.8卷,11期(2009-11-01):4943-4953()
  15. Chen SF、Juang YL、Chou WK、Lai JM、Huang CY、Kao CY、Wang FS,「 Inferring a transcriptional regulatory network of the cytokinesis-related genes by network component analysis.」,BMC Syst Biol.27卷,3期(2009-11-01):110()
  16. Wu CC、 Yu CT、 Chang GC、Lai JM、 Hsu SL,「Aurora-A promotes gefitinib resistance via a NF-κB signaling pathway in p53 knockdown lung cancer cells.」,Biochem Biophys Res Commun.405卷,2期(2011-01-01):168-172(SCI)
  17. Mei-Chi Shih、Jean-Yi Chen、Yu-Chung Wu、Yih-Hua Jan、Bo-Min Yang、Pei-Jung Lu、Hui-Chuan Cheng、Chih-Jen Yang、Michael Hsiao、Jin-Mei Lai,「TOPK/PBK promotes cell migration via modulation of the PI3K/PTEN/AKT pathway and is associated with poor prognosis in lung cancer」,Oncogene31卷,19期(2012-05-01):2389-2400(SCI)
  18. Chen MH、Yang WL、Lin KT、Liu CH、Liu YW、Huang KW、Chang PM、Lai JM、Hsu CN、Huang CY,「Gene expression-based chemical genomics identifies potential therapeutic drugs in hepatocellular carcinoma.」,PLoS One6卷,11期(2011-11-01):e27186-e27195(SCI)
  19. Wei DC、Yeh YC、Hung JJ、Chou TY、Wu YC、Lu PJ、Cheng HC、Hsu YL、Kuo YL、Lai JM,「Overexpression of TOPK predicts poor prognosis in patients with stage I lung adenocarcinoma」,Cancer Science103卷,4期(2012-04-01):731-738(SCI)
  20. Cheng CC、Wu LC、Lai JM、Chen CT、Hsueh CM、Hsu SL,「Ethanol extract of Graptopetalum paraguayense upregulates paraoxonase 1 gene expression via an AKT/NF-κB-dependent pathway.」,Am J Chin Med40卷,2期(2012-03-01):357-372(SCI)
  21. Yeh CT、Wu AT、Chang PM、Chen KY、Yang CN、Yang SC、Ho CC、Chen CC、Lai JM、Huang CY,「Trifluoperazine, an antipsychotic agent, inhibits cancer stem cell growth and overcomes drug resistance of lung cancer.」,American Journal of Respiratory and Critical Care Medicine186卷,11期(2012-12-01):1180-1188(SCI)
  22. Su LJ、Chang CC、Yang CH、Hsieh SJ、Wu YC、Lai JM、Tseng TL、Huang CY、Hsu SL,「Graptopetalum paraguayense ameliorates chemical-induced rat hepatic fibrosis in vivo and inactivates stellate cells and kupffer cells in vitro.」,PLoS One8卷,1期(2013-01-01):e53988(SCI)
  23. Chen TC、Liu YW、Huang YH、Yeh YC、Chou TY、Wu YC、Wu CC、Chen YR、Lai JM、Huang CY,「Protein Phosphorylation Profiling Using an In Situ Proximity Ligation Assay: Phosphorylation of AURKA-Elicited EGFR-Thr654 and EGFR-Ser1046 in Lung Cancer Cells」,PLoS One.8卷,3期(2013-03-01):e55657(SCI)

會議論文

  1. 賴金美,「Novel regulators in cytokinesis」,ASCB 45th Annual Meeting,San Francisco,America,,2005-12-10
  2. Mei-Chi Shih、 Jean-Yi Chen、Jin-Mei Lai,「Functional characterization of TOPK in lung carcinogenesis」,BIT’s Annual World Cancer Congress-2008,Shanghai,China,,2008-06-12
  3. 賴金美、陳貞伊,「Elucidation the function of TOPK/PBK by identifying it’s interacting protein via yeast two-hybrid screen.」,生醫年會,,臺灣,,2007-03-24
  4. 賴金美、楊博閔,「Promoter methylation profiling of seven down-regulated genes in female lung adenocarcinoma.」,生醫年會,,臺灣,,2007-03-24
  5. 賴金美、施美琪,「Identification and Characterization of TOPK Interaction Protein by GST-TOPK Pull Down and LC/Mass/Mass Analysis」,生醫年會,,臺灣,,2008-03-29
  6. 賴金美、夏瑞遠,「Elucidation the role of a novel down-regulated gene, FAM134B, in lung adenocarcinoma.」,生醫年會,,臺灣,,2009-03-28
  7. 賴金美、陳韋仲,「Investigation the role of KIAA0101 in cancer metastasis」,生醫年會,,臺灣,,2009-03-28
  8. 賴金美、陳奕君,「Functional Characterization of the Interactome of TRIM37」,生醫年會,,臺灣,,2009-03-28
  9. Jin-Mei Lai、Mei-Chi Shih,「From Integrated Microarray Analyses to Novel Metastasis Associated Gene Identification」,BIT Life Sciences’ 2nd World Cancer Congress,,Beijing, China,,2009-06-22
  10. 魏迪卿、賴金美,「探討TOPK/PBK 所誘導之細胞移行機制及其在癌症治療上所扮演之潛在角色」,生醫年會,,臺灣,,2011-03-19
  11. 蕭婷分、賴金美,「Identification and characterization of potential Wnt signaling inhibitors」,生醫年會,,臺灣,,2012-03-17

專書

  1. Lai JM,The involvement of LPA/RhoA/ROCK signaling pathway in Phorbol-12-Myristate-13-Acetate-induced survival and death(:,2003-02-01)

國科會研究計畫



  1. 賴金美,TOPK磷酸激酶與PTPmu去磷酸酶於肺腺癌形成的角色探討 ,2006-08-01~2009-07-31,NSC 95-2311-B-030-002-MY3
  2. 賴金美、黃奇英、高成炎、王逢盛,中心顆粒體之蛋白質交互作用網路-(子計畫三)以酵母菌雜交系統探討中心顆粒體之蛋白質交互作用網絡(1/3),2006-08-01~2007-07-31,NSC 95-2627-B-030-001-
  3. 賴金美,PKC與RhoA/ROCK主導的訊息調節對於造血細胞命運決定之角色探討,2004-10-01~2005-07-31,NSC 93-2311-B-030 -002
  4. 賴金美,以caspase prodomain建立分離促細胞走向凋亡的蛋白質複合體,2005-07-01~2006-02-28,NSC
  5. 賴金美,RhoA/ROCK主導的肌動蛋白重整與細胞淍亡機制之啟動探討,2005-08-01~2006-07-31,NSC 94-2311-B-030-002
  6. 賴金美、黃奇英、高成炎、王逢盛,中心顆粒體之蛋白質交互作用網路-(子計畫三)以酵母菌雜交系統探討中心顆粒體之蛋白質交互作用網絡,2007-08-01~2008-07-31,NSC 96-2627-B-030-001
  7. 賴金美、黃奇英、高成炎、王逢盛,中心顆粒體之蛋白質交互作用網路-(子計畫三)以酵母菌雜交系統探討中心顆粒體之蛋白質交互作用網絡(3/3),2008-08-01~2009-07-31,NSC97-2627-b030-001
  8. 賴金美、黃奇英、王逢盛,藉由蛋白質交互作用網路重建KEGG的訊息傳遞網路-(子計畫三)-尋找合併療法標的關鍵蛋白質交互作用(1/3),2010-08-01~2011-07-31,NSC 99-2627-B-030-001
  9. 賴金美、黃奇英、王逢盛,藉由蛋白質交互作用網路重建KEGG的訊息傳遞網路-(子計畫三)-尋找合併療法標的關鍵蛋白質交互作用(2/3),2011-08-01~2012-07-31,NSC
  10. 賴金美,評估TOPK於DNA損傷反應及肺癌治療之角色,2012-08-01~2013-07-31,NSC 101-2320-B-030-009-
  11. 賴金美,藉由蛋白質交互作用網路重建KEGG的訊息傳遞網路-(子計畫三)-尋找合併療法標的關鍵蛋白質交互作用(3/3),2012-08-01~2013-07-31,NSC 101-2627-B-030-001
  12. 賴金美,網絡醫學新契機:解析肝癌之標的蛋白質交互作用及代謝網路-肝癌治療的潛力策略從脂肪生成的訊息途徑著手(子計畫3)(1/3) ,2013-08-01~2014-07-31,NSC102-2627-B-030-001

非國科會研究計畫



  1. 賴金美,血球前趨細胞得生長分化與細胞骨架蛋白質的相關訊息調控,輔仁大學,2004-12-01~2005-06-30
  2. 賴金美,利用功能性基因體學探討在肺癌形成時可能的抑癌基因之角色扮演,台中榮總專題研究計畫(子計畫),TCVGH-937317D,2005-01-01~2005-12-31,TCVGH-947325D
  3. 賴金美,利用功能性基因體學探討在肺癌形成時可能的抑癌基因,PTPRG 和PTPRM,之角色,台中榮總,2006-01-01~2006-12-31,TCVGH-957328D
  4. 賴金美,探討肺腺癌低量表現基因MOSC2之基因表現調控。,台中榮總,2008-01-01~2008-12-31,TCVGH-977328D
  5. 賴金美,探討新穎基因FLJ20152與肺癌形成的角色。,台中榮總,2007-01-01~2008-12-31,TCVGH-967328D
  6. 賴金美、徐士蘭,保肝產品的新契機:探討從抗肝纖維化藥物萃取之高活性分子對抗肝癌的效用 ,經濟部,2009-06-01~2010-05-31,98EC17A17S20145

會議論文

  1. 陳貞伊,「Elucidation the function of TOPK/PBK by identifying it’s interacting protein via yeast two-hybrid screen.」,生醫年會,,臺灣,,2007-03-24
  2. 楊博閔,「Promoter methylation profiling of seven down-regulated genes in female lung adenocarcinoma.」,生醫年會,,臺灣,,2007-03-24
  3. 施美琪,「Identification and Characterization of TOPK Interaction Protein by GST-TOPK Pull Down and LC/Mass/Mass Analysis」,生醫年會,,臺灣,,2008-03-29
  4. 夏瑞遠,「Elucidation the role of a novel down-regulated gene, FAM134B, in lung adenocarcinoma.」,生醫年會,,臺灣,,2009-03-28
  5. 陳韋仲,「Investigation the role of KIAA0101 in cancer metastasis」,生醫年會,,臺灣,,2009-03-28
  6. 陳奕君,「Functional Characterization of the Interactome of TRIM37」,生醫年會,,臺灣,,2009-03-28
  7. 施美琪,「From Integrated Microarray Analyses to Novel Metastasis Associated Gene Identification」,BIT Life Sciences’ 2nd World Cancer Congress,,Beijing, China,,2009-06-22
  8. 魏迪卿,「探討TOPK/PBK 所誘導之細胞移行機制及其在癌症治療上所扮演之潛在角色」,生醫年會,,臺灣,,2011-03-19
  9. 蕭婷分,「Identification and characterization of potential Wnt signaling inhibitors」,生醫年會,,臺灣,,2012-03-17

指導碩博士論文

  1. 賴金美,陳信賓,碩士,94,「利用酵母菌雙合雜交及肺腺癌組織之序列分析以探討FLJ20152基因之功能」
  2. 賴金美,陳貞伊,碩士,95,「利用酵母菌雙合雜交系統找尋交互作用之蛋白質以探討TOPK/PBK磷酸激酶之功能」
  3. 賴金美,楊博閔,碩士,95,「評估CpG island的甲基化程度與女性肺腺癌中低量表現基因之關聯」
  4. 賴金美,傅綜信,碩士,95,「肺腺癌低量表現基因MOSC2(Molybdenum cofactor sulfurase C-terminal containing2)之功能性探討」
  5. 賴金美,施美琪,碩士,96,「利用GST-TOPK pull down與LC/Mass/Mass找尋與鑑定TOPK結合之蛋白質並進行功能探討」
  6. 賴金美,陳韋仲,碩士,97,「研究KIAA0101於癌症轉移中扮演之角色」
  7. 賴金美,夏瑞遠,碩士,97,「肺腺癌低表現量基因FAM134B之功能探討」
  8. 賴金美,陳奕君,碩士,98,「藉由相結合的TOPK和HURP對TRIM37進行功能性探討」
  9. 賴金美,徐瑜臨,碩士,99,「探討PI3K-PTEN-AKT訊息傳遞路徑於TOPK誘導之細胞移行」
  10. 賴金美,魏迪卿,碩士,99,「探討TOPK/PBK 所誘導之細胞移行機制及其在癌症治療上所扮演之潛在角色」
  11. 賴金美,蕭婷分,碩士,100,「Identification and characterization of potential Wnt signaling inhibitors」
  12. 賴金美,陳冠潔,碩士,101,「探討TOPK於順鉑誘發肺癌細胞毒殺時所扮演的角色」

獲獎紀錄

  1. 賴金美,95學年度,輔仁大學研究發展處,獎勵教師申請校外研究計劃補助
  2. 賴金美,96學年度,輔仁大學研究發展處,獎勵教師申請校外研究計劃補助
  3. 賴金美,97學年度,輔仁大學研究發展處,獎勵教師申請校外研究計劃補助
  4. 賴金美,99學年度,輔仁大學研究發展處,輔仁大學專任教師及研究人員投稿國際期刋學術論文獎勵
  5. 賴金美,97學年度,輔仁大學研究發展處,輔仁大學專任教師及研究人員投稿國際期刋學術論文獎勵
  6. 賴金美,98學年度,輔仁大學研究發展處,輔仁大學專任教師及研究人員投稿國際期刋學術論文獎勵
  7. 賴金美,96學年度,輔仁大學研究發展處,教師及研究人員研究成果獎勵
  8. 賴金美,94學年度,輔仁大學研究發展處,獎勵教師申請校外研究計劃補助
  9. 賴金美,100學年度,國科會,國科會100年度特殊優秀人才獎勵

演講

  1. 賴金美,From Integrated Microarray Analyses to Novel Metastasis Associated Gene Identification,Beijing, China,BIT Life Science’2nd World Cancer Congress,2009-06-22
  2. 賴金美,From Integrated Microarray Analyses to Novel Metastasis Associated Gene Identification,Dalian, China ,BIT's 4th World Congress of Cancer ,2011-05-23
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